Coding
Part:BBa_K1945005:Design
Designed by: Sharon Lian Group: iGEM16_CSU_Fort_Collins (2016-10-21)
C-terminal fragment of T7 RNA Polymerase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The C-terminal sequence of the T7 RNA polymerase from bacteriophage T7. Paired with the N-terminal sequence, this can create an AND gate.
Source
From bacteriophage T7.
References
The idea to use a split T7 RNA polymerase to create an AND gate was inspired by Shis, D. L., & Bennett, M. R. (2013). Library of synthetic transcriptional AND gates built with split T7 RNA polymerase mutants. Proceedings of the National Academy of Sciences, 110(13), 5028-5033. doi:10.1073/pnas.1220157110