Coding

Part:BBa_K1945005:Design

Designed by: Sharon Lian   Group: iGEM16_CSU_Fort_Collins   (2016-10-21)


C-terminal fragment of T7 RNA Polymerase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The C-terminal sequence of the T7 RNA polymerase from bacteriophage T7. Paired with the N-terminal sequence, this can create an AND gate.

Source

From bacteriophage T7.

References

The idea to use a split T7 RNA polymerase to create an AND gate was inspired by Shis, D. L., & Bennett, M. R. (2013). Library of synthetic transcriptional AND gates built with split T7 RNA polymerase mutants. Proceedings of the National Academy of Sciences, 110(13), 5028-5033. doi:10.1073/pnas.1220157110